The situation happens in a Dissolved oxygen chamber, as part of polarography exp

Question

The situation happens in a Dissolved oxygen chamber, as part of polarography experiment. We have isolated mitochondria in the chamber.

1) With glutamate in the chamber what should happen to the rate of oxygen consumption after adding rotenone?
A. it should speed up B. it should slow down C. it should stay the same
2) Suppose you have glutamate in the chamber and have added rotenone. Now you add ADP. What should happen to the rate of oxygen consumption?
A. it should speed up B. it should slow down C. it should stay the same 3) Suppose you have glutamate in the chamber along with mitochondria and no poisons. Respiration is taking place. What happens to oxygen consumption if you add NADH? A. it should speed up B. it should slow down C. it should stay the same
B. it should slow down C. it should stay the same C. it should stay the same C. it should stay the same 3) Suppose you have glutamate in the chamber along with mitochondria and no poisons. Respiration is taking place. What happens to oxygen consumption if you add NADH? A. it should speed up B. it should slow down C. it should stay the same
C. it should stay the same

Explanation / Answer

1) With glutamate in the chamber, the rate of oxygen consumption after adding rotenone B. it should slow down 2) With glutamate in the chamber and have added rotenone. Adding ADP the rate of oxygen consumption A. it should speed up 3) With glutamate in the chamber along with mitochondria and no poisons. Respiration is taking place. oxygen consumption after adding NADH C. it should stay the same

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