Chromatography As the name suggests, planar chromatography works in a plane, tha

Question

Chromatography

As the name suggests, planar chromatography works in a plane, that is the stationary phase is set on a plane such as paper or a plate. For example, in a thin layer chromatography (tlc) method, the stationary phase is coated on a plate and the mixture to be separated is spotted on the plate. It is placed and run in a chamber with mobile phase and the compounds gets separated on the plate. Similarly the paper chromatography works but on a paper. The method is fast, works with multiple types of compounds, uses relatively smaller amounts of mobile phase and identification of compounds is done directly on the plate.

In column chromatography, the stationary phase is loaded into a column with the help of the mobile phase. The compound is then loaded on top of it and sepration is done using a large volume of solvents (mobile phase). The method is relatively slower than planar chromatography. Works with all types of compounds. Can be scaled up or down depending on the amount of mixture to be separated.

Explanation / Answer

Q1 (8 points) Answer the following questions: a. Compare between the planar and column chromatography methods and validate your answer with examples of instrumentation.

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