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The reaction that converts oxaloacetic acid (OAA) to phosphoenol pyruvate (PEP)

ID: 78431 • Letter: T

Question

The reaction that converts oxaloacetic acid (OAA) to phosphoenol pyruvate (PEP) is an essential reaction in gluconeogenesis. The list below contains various compounds that are involved directly or indirectly in this OAA to PEP step. For ANY EIGHT items in this list, (a), name one compound or structure to which it binds when it functions in this process and (b). describe the function relating to the OAA to PEP step it carries out. Note that at least one of the compounds listed below does NOT participate in this process, and for each of them you should write NONE in the "Function" column. An example is show for glucagon below.

Explanation / Answer

ANSWER:

COMPOUNDS:

1) CORTISOL

BINDS TO    Glucocorticoid Receptor

FUNCTION    passes through the lipid membrane of liver cells

2) CREB   

BINDS TO      CREB then binds upstream of the PEPCK-C  gene at CRE and induces PEPCK-C transcription

  FUNCTION    PEPCK-C by increasing the levels of cAMP in the liver which consequently leads to the phosphorylation of S133 on a beta sheet in the CREB protein.

3)GRE

BINDS TO PEPCK-C

FUNCTION   This receptor dimerizes and the cortisol/GR complex passes into the nucleus where it then binds to the Glucocorticoid Response Element.

4) cAMP

BINDS TO NONE

FUNCTION Glucagon indirectly elevates the expression of PEPCK-C by increasing the levels of cAMP.

5) Glucocoticoid Receptor

BINDS TO Glucocoticoid Receptor

FUNCTION Is composed from its role in regulation of glucose metabolism, synthesis in the adrenal cortex, and its steroidal structure

6) PEP-CK

BINDS TO NONE

FUNCTION   It converts oxaloacetate intophosphoenolpyruvate and carbon dioxide. It is found in two forms, cytosolic and mitochondrial.

7) RIBOSOMES

BINDS TO None

FUNCTION

8) Lac Repressor

BIMDS TO None

FUNCTION

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