What following method can be used to grow O_2-sensitive, obligated anaerobes? a.

Question

What following method can be used to grow O_2-sensitive, obligated anaerobes? a. MSA plates b. Thioglycollate broth c. TSB d. EMB plates ____are strains that are unable to grow on a minimal medium that supports growth of the wild type strain, but the minimal medium if one or more nutritional supplements are can grow on added. a. Prototrophs b. Autotrophs c. Auxotrophs d. Minitrophs A microbiologist is working with two genera of bacteria, A and B, to determine if cells are competent. Strains are grown under optimal conditions, and DNA fragments that the gene for green fluorescent protein are added to each culture at 2 minute intervals. Samples from each treatment are plated on a nutrient agar and incubated, after which the plates are examined under an ultraviolet lamp. The microbiologist observes that all plates of bacteria B contain colonies that are fluorescent, while for bacteria A only the plates from stationary phase demonstrate fluorescent colonies. What can be concluded from these results? a. Competence in bacteria B is dependent on the phase of growth. b. Bacteria A is competent in the stationary phase of growth. c. Competence in bacteria A is constitutively expressed. d. Neither bacteria A nor bacteria B cells are competent. A plasmid, carrying a desired gene with an antibiotic gene, was used for transformation. The antibiotics incorporated into the culture medium can containing the desired gene. a. select against organisms that have incorporated a plasmid b. select for organisms that have incorporated a plasmid not containing the desired gene c. enhance production of recombinant proteins.

Explanation / Answer

12 ans- B thioglycollate broth

is a differential media used to determine oxygen requirerments of micro-organism or aerotolerance bacteria.obligated anerobes are poisoned by oxygen so they collect at the bottom of the tube where oxygen concentration are low.

13 ans-C-Auxotrophic bacteria

Bacteria will not grow on minimal medium but can grow in minimal media if one or more nutritional suplements or requiring a specific growth substance are added.

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