Humans are aerobic organisms if so why do we worry about being infected by an an

Question

Humans are aerobic organisms if so why do we worry about being infected by an anaerobic organism? A swab for culture is received from a doctor's office. The staff forgot to mention the source from where the swab was taken and the clinic was closed for next few days. What different selective/differential media may be helpful to you to identify the organism. Explain very briefly your rationale for using the particular culture media.? Agar and gelatin are used as a solidifying agent for bacteriological media. Why is gelatin less suitable than the agar? A test is performed on an isolated colony on a blood agar plate and bubbles appear. The test is repeated on another isolated colony form the same plate. This lime there are no bubbles. How can you explain the difference in the results? You are given a rube of contaminated water. The number of bacteria in the tube is too many 10 count. You prepare a tenfold dilutions in 5 tubes, so that tube #1 has 1:10 dilution, tube #2 has 1:100 dilution, tube #3 has 1:1000 dilution, tube #4 has 1:10,000 dilution and tube #5 has 1:100,000 dilution. The second tube has 210 cells and the third tube has 26 colonies. What is the number of bacteria per ml in the original sample? Several individuals have food borne poisoning due to Salmonella from a common source. Later several individuals went to the local emergency rooms of the hospital complaining of similar symptoms. How could you determine and be sure that these individuals have the same infection? Distinguish between alpha-and beta? How would you differentiate between Staph and Stress? Why are females more prone to UT than males? What would be the effect of a urine sample being left at room temperature overnight before transported to the laboratory for culture?

Explanation / Answer

1. anaerobic bacteria are common cause of infection, some of which can be serious or life threatening. Because anaerobes are predominant components of the flora of the skin or mucous membranes, they are a common cause infections of endogenous origin. Because of their fastidious nature, anaerobes are hard to culture and isolate and are often not recovered from infected sites.

2. Blood agar is used because it is used to grow those organism which do not grow easily mostly fastidious bacterias. Other reason for using this media is it is used to grow wide range of culture.

3. Agar is preferred in media because agar stay solid at higher temperatures than gelatin and the other reason is that bacteria can digest gelatin more readily than agar.

4. presence of bubbles means the oxygen is released in the culture,it means isolated bacteria are able to produce catalase enzyme.so, they are strict aerobic or facultative anaerobic bateria. And the media on which oxygen bubbles are absent they are anaerobic bateria.

7. alpha hemolysis is also called as partial hemolysis. It is partial lysis of red cells in the media which produce green oxidized derivative methamoglobin while beta hemolysis called as complete hemolysis. It is complete lysis of red cell in the media around and under the colonies.

9. Females are more prone to UTI than males because:

Related Questions

Navigate

• Browse (All)
• Browse H
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.