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When an unknown mixture\'s peaks are too close together and eluted in the range

ID: 995757 • Letter: W

Question

When an unknown mixture's peaks are too close together and eluted in the range k = 1-3, what change in the solvent composition would you make in each of the following situations, and why? Reversed phase column using 50% tetrahydrofuran/50% water. Lower concentration of tetrahydrofuran to lower solvent strength. Lower concentration of tetrahydrofuran to increase solvent strength. Increase concentration of tetrahydrofuran to lower solvent strength. Increase concentration of tetrahydrofuran to increase solvent strength. Normal phase column using 50% hexane/50% diethyl ether. Lower concentration of hexane to lower solvent strength. Lower concentration of hexane to increase solvent strength. Increase concentration of hexane to lower solvent strength. Increase concentration of hexane to increase solvent strength.

Explanation / Answer

a) 3rd answer is correct.

b) 2nd answer is correct

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