When an unknown mixture\\\'s peaks are too close together and eluted in the rang

Question

When an unknown mixture's peaks are too close together and eluted in the range k = 1–3, what change in the solvent composition would you make in each of the following situations, and why?

When an unknown mixture's peaks are too close together and eluted in the range 1-3, what change in the solvent composition would you make in each of the following situations, and why? (a) Reversed phase column using (b) Normal phase column usin 50% tetrahydrofuran/50% water 50% hexane/50% diethyl ether Lower concentration of hexane to lower solvent strength Lower concentration of hexane to increase solvent strength Increase concentration of hexane to lower solvent strength Increase concentration of hexane to increase Lower concentration of tetrahydrofuran to lower solvent strength O Lower concentration of tetrahydrofuran to increase solvent strength Increase concentration O of tetrahydrofuran to lower solvent strength O Increase concentration of tetrahydrofuran to increase solvent strength solvent strength

Explanation / Answer

a) In a reversed phase column, polar compounds will come first followed by nonpolar compounds. So lower the concentration of tetrahydrofuran to increase solvent strength to get more polar first followed by less polar compound.

b) In a normal phase column, nonpolar compounds will come first followed by polar compounds. So increase concentration of hexane to lower solvent strength to get less polar first followed by more polar compound.

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