Cell biology 22. Hsp70 and TriC represent this class of proteins that help preve

Question

Cell biology 22. Hsp70 and TriC represent this class of proteins that help prevent nascent proteins from participating in inappropriate interactions during folding. A) Hub proteins B) Denaturases C) Conformases E) B and C 23. Which of the following is/are properties of enzymes? ) Highly specific for reactants Il) Not permanently altered during the course of the reaction III) Only affect the rates, not the IV) Only present in the cell in small amounts of reactions A) I B) I C),III, IV 24. This class of amino acids contain R groups that act as weak organic acids and bases, and can readily form hydrogen bonds A) Polar uncharged B) Polar charged C) Non-polar D) Side chains with unique properties (e.g. glycine, proline, cysteine) E) A and B 25. Given the Lineweaver-Burk plot, for the uninhibited enzyme (line A) you have the following values: X-intercept of -4.5 and Y-intercept of 0.25. Calculate the corresponding Vmax for the uninhibited enzyme. A) 0.25 C) 2 D) 4 E) 8 1/N 26. Calculate the corresponding KM for the uninhibited enzyme A)-0.222 1/S C) 0.111 D) 0.222 E) 0.25 27. Which line represents the use of a competitive inhibitor? A) Line A B) Line B C) Line C 28. For the metabolic reaction below, the most probable mechanism of allosteric modulation would be that molecule acts through feedback inhibition on enzyme A+BC (Enzyme AB) CDE (Enzyme CD)E+FG (Enzyme EF) A) E; EF B) G: EF C) E: AB D) G: AB E) C: EF

Explanation / Answer

22. Chaperones.

Molecular chaperones are multidomain proteins evolved to assist nascent proteins to reach their native fold, protect subunits from heat shock during the assembly of complexes, prevent protein aggregation or mediate targeted unfolding or disassembly.

23.All are true.

Enzymes are present in small amounts in the cell because enzymes are not consumed as a result of the reaction they catalyze. So a small amount of enzymes can be reused to catalyze a large no of reactions.

Enzymes are highly specific both in the reactions that they catalyze and in their choice of reactants, called substrates.

Enzymes are not permanently altered. There are specific enzymes for specific chemical reactions. They are not altered during the process because they can be reused at the end of the reaction.

24.

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