What is the function of LacI and cap protein? How are these 2 signals integrated
ID: 260323 • Letter: W
Question
What is the function of LacI and cap protein? How are these 2 signals integrated for signaling for lactose? What would happen if there was a mutation on lacI, operator, repressive, cap protein? I know there’s something about glucose too but I need help figuring it all out!! What is the function of LacI and cap protein? How are these 2 signals integrated for signaling for lactose? What would happen if there was a mutation on lacI, operator, repressive, cap protein? I know there’s something about glucose too but I need help figuring it all out!!Explanation / Answer
I will try to be as brief as possible:
Lacl is a gene in lac operon that codes for the protein that acs as a repressor of the lac operon by binding to the operator region and hence stopping RNA polymerase from binding and create RNA transcripts.
CAP protein is a protein that binds to the cap site that is present just before the promoter region and binding of this protein helps RNA polymerase bind effeciently to the promoter region.
If there is a mutation of lacl, the repressor would not be formed and the lac operon would be constitutively expressed throughout the organism's lifetime.
If operator is mutated, the repressor would no longer able to bind to this region which would again lead to constitutive expression.
If there is a mutation in CAP protein, it would not bind to the cap region and hence will not be able to promote polymerase binding. So, the expression level of Lac operon would be very low.
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