In paper chromatograph what makes the components(example pen ink) travel up the
ID: 862161 • Letter: I
Question
In paper chromatograph what makes the components(example pen ink) travel up the paper and how does the solvent being used effect if the components seperate at all or how well they seperate?And also could you please define and explain the differences between mobile and stationary phase.
Thank you so much! In paper chromatograph what makes the components(example pen ink) travel up the paper and how does the solvent being used effect if the components seperate at all or how well they seperate?
And also could you please define and explain the differences between mobile and stationary phase.
Thank you so much!
And also could you please define and explain the differences between mobile and stationary phase.
Thank you so much!
Explanation / Answer
In paper chromatography substances are distributed between a stationary phase and a mobile phase.
The stationary phase. It is a piece of high quality filter paper.
The mobile phase It is a developing solution that travels up the stationary phase, carrying the samples with it. Components of the sample will separate according to how strongly they adsorb onto the stationary phase versus how readily they dissolve in the mobile phase.
When a colored chemical sample is placed on a filter paper, the colors separate from the sample by placing one end of the paper in a solvent. The solvent diffuses up the paper, dissolving the various molecules in the sample according to the polarities of the molecules and the solvent. If the sample contains more than one colour, that means it must have more than one kind of molecule.
Because of the different chemical structures of each kind of molecule, the chances are very high that each molecule will have at least a slightly different polarity, giving each molecule a different solubility in the solvent. The unequal solubilities cause the various color molecules to leave solution at different places as the solvent continues to move up the paper. The more soluble a molecule is, the higher it will migrate up the paper.
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