At pH 7, Protein P has a positive charge and Protein N has a negative charge. Wh

Question

At pH 7, Protein P has a positive charge and Protein N has a negative charge. Which of the following statements is TRUE regarding how these proteins bind to a column in ion-exchange chromatography? Binding of either protein to the column will not change much if we change the pH of the solution. Either cation-exchange or anion-exchange chromatography could be successfully used to separate Protein P from Protein N. A high concentration of free salt in the solution will make both of these proteins bind more tightly to the column. Protein P will bind more tightly than Protein N to an anion-exchange column. All of the above are false.

Explanation / Answer

Either cation exchange chromatography or anion chamge chromatography could be successfully used to separate protein P from Protein N.

The cation exchange chromatography retains positively charged cations the stationary phase being the negatively charged functional group.

Anion exchange chromatigraphy retains negatively charged anions, the stationary phase being a postively charged functional group.

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