At pH 7, Protein P has a positive charge and Protein N has a negative charge. Wh

Question

At pH 7, Protein P has a positive charge and Protein N has a negative charge. Which of the following statements is TRUE regarding how these proteins bind to a column in ion-exchange chromatography? Binding of either protein to the column will not change much if we change the pH of the solution. Either cation-exchange or anion-exchange chromatography could be successfully used to separate Protein P from Protein N. A high concentration of free salt in the solution will make both of these proteins bind more tightly to the column. Protein P will bind more tightly than Protein N to an anion-exchange column. All of the above are false.

Explanation / Answer

option A2 is correct.

When an ion exchange chromatography column is loaded with a sample at a particular pH,the protein N that carries a net negative charge binds to a positively charged anion exchange resin which captures this protein. The protein P, carries a net positive charge, thus a negatively-charged cation exchange resin is chosen to capture this protein.Ion exchange chromatography resins are composed of positively or negatively charged functional groups that are covalently bound to a solid matrix.To elute proteins from an anion exchange resin, a decreasing pH gradient is chosen, while an increasing pH gradient is chosen for elution from cation exchangers

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