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Chromatography In order to maintain the quality of their packaged products, the

ID: 910988 • Letter: C

Question

Chromatography

In order to maintain the quality of their packaged products, the Murdoch Tea and Coffee Company regularly conducts HPLC analysis of their raw ingredients to determine the levels of certain antioxidants and the caffeine content. Due to a fault in the HPLC software, results from analyses are being displayed as a data table instead of an easy to read chromatogram (b) Describe what you could do to reduce the overall analysis time for the analysis? Explain why this approach is likely to work. (d) A UV-visible detector can be used to detect caffeine due to its strong chromophore. Describe a detector, including how it works, that could be used to detect a non-charged organic compound that does not have a UV-visible chromophore.

Explanation / Answer

d) Refractive Index Detector: Generally called as RI Detector or Universal detector

In which the sample will be detected by its specific refractive index property, even it is non-charged organic campound without any chromophore.

The Refractive index, where the deflection of the light will be changed when the composition in the sample is changed againist the reference material

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