Copy and paste the following nucleotide sequence into the “Enter Query Sequence”

Question

Copy and paste the following nucleotide sequence into the “Enter Query Sequence” search box on the NCBI BLAST site (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&P AGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome). Under the “Choose Search Set” heading, select the Others (nr etc.) button and click on the BLAST button near the bottom of the page. The results page may take a few minutes to load. Use the information to answer questions A through E.

GGCCGCGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGGCGGC GGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCAGG CACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGTA CGGAGCGGACCACCCCTCCTGGGCCCCTGCCCGGGTCCCGACCCTCTTTGCCGGCGCCG GGCGGGGCCGGCGGCGAGTGAATGAATTAGGGGTCCCCGGAGGGGCGGGTGGGGGGC GCGGGCGCGGGGTCGGGGCGGGCTGGGTGAGAGGGGTCTGCAGGGGGGAGGCGCGCG GACGCGGCGGCGCGGGGAGTGAGGAATGGGCGGTGCGGGGCTGAGGAGGGTGAGGCT GGAGGCGGTCGCCGCTGGTGCTGCTTCCTGGACGGGGAACCCCTTCCTTCCTCCTCCCC GAGAGCCGCGGCTGGAGGCTTCTGGGGAGAAACTCGGGCCGGGCCGGCTGCCCCTCGG AGCGGTGGGGTGCGGTGGAGGTTACTCCCGCGGCGCCCCGGCCTCCCCTCCCCCTCTCC CCGCTCCCGCACCTCTTGCCTCCCTTTCCAGCACTCGGCTGCCTCGGTCCAGCCTTCCCT GCTGCATTTGGCATCTCTAGGACGAAGGTATAAACTTCTCCCTCGAGCGCAGGCTGGACG GATAGTGGTCCTTTTCCGTGTGTAGGGGATGTGTGAGTAAGAGGGGAGGTCACGTTTTGG AAGAGCATAGGAAAGTGCTTAGAGACCACTGTTTGAGGTTATTGTGTTTGGAAAAAAATGC ATCTGCCTCCGAGTTCCTGAATGCTCCCCTCCCCCATGTATGGGCTGTGACATTGCTGTGG CCACAAAGGAGGAGGTGGAGGTAGAGATGGTGGAAGAACAGGTGGCCAACACCCTACAC GTAGAGCCTGTGACCTACAGTGAAAAGGAAAAAGTTAATCCCAGATGGTCTGTTTTGCTTG GTCAAGTTAAACCCGAAGAAAACCCGCAGAGCAGAAGCAAGGCTTTTTCCTTGCTAGTTGA GTGTAGACAGCAATAGCAAAAATAGTACTTGAAGTTTAATTTACCTGTTCTTGTCCTTTCCC CTATTTCTTATGTATTACCCTCATCCCCTCGTCTCTTTTATACTACCCTCATTTTGCAGATGT GTTCTACATCTCAAGAGTTATTACAGTACTCCAAAACAGCACTTACATGATTTTTTAAACTTA CAGAGGAATTGTAGCAATCCACCAGCTAACCGCCTGAAATAGACTTAAACATGTGCATCTC CTTTTTTTTTTTTTTTTTGAGACACAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAATGGCG CGGTATCGGCTCACTGAAACCTCCGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTC CCGAGTAGCTGGGACTAGTAGGTGCACGCCACCATGCCCAGCTAATTTTTGTATTTTTAGT AGAGACAGAGTTTCATCATGTTGGTCAGGATGGTCTCCATCTGCTCTGTTGCCCAGGCTGG AGTGCAGTGGCGCCGTCTCGGCTCACTGCAACCTCTGCCTCCTGCATTCAAGCAATTCTC CTGCCTCAGCCTCCCGAATAACTGGGATTACAGGTGTCTGCTGCCATGCCCGGCTAATTTT TTGTATTTTTAGTAGAGACGGGGGTTTCACCATGTTGGTCAGGCTGGTCTAGAACTCCTGA CCTCGTGATCTGCCCGCCTCGGCCTCCCACAGTGGCATGTGCATCTTATAGCTGAAGTCTA AGCCTTCTTAAATCTTGAGAT

A. Based on the first few links under the “Descriptions” heading on the Results page, what is the name of the sequence containing these nucleotides?

B. The name of the sequence also refers to the name of the protein – what is the function of this protein and how does it relate to cancer development? Be specific and relate to previous aspects of the course

C. What organism contains this sequence?

D. Scanning down the list, what other organism contains this gene? Is this surprising or not?

E. Identify a primer pair that may be used in a PCR to amplify the entire sequence above. Each primer should be 20 nucleotides long and be written in the 5’ to 3’ direction.

Explanation / Answer

A. Based on the first few links under the “Descriptions” heading on the Results page, what is the name of the sequence containing these nucleotides?

Homo sapiens KRAS proto-oncogene, GTPase (KRAS), RefSeqGene on chromosome 12

The name of the sequence also refers to the name of the protein – what is the function of this protein and how does it relate to cancer development? Be specific and relate to previous aspects of the cours

KRAS protein

What organism contains this sequence?

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