Given the following mRNA: 5\'-AUG-AAA-UCU-CCC-CGU-UGG-UAU-AUA-UUU-UGA-3\' Please

ID: 176455 • Letter: G

Question

Given the following mRNA: 5'-AUG-AAA-UCU-CCC-CGU-UGG-UAU-AUA-UUU-UGA-3'
Please answer all 1a. - 1f.
1a. Write the peptide coded by this mRNA strand. 1b. Write a different mRNA strand which codes for the same peptide, but has four point mutations. 1c. If, in the original strand, A5 is replaced by a G, and U7 is replaced by an C, what peptide is produced? 1e. If, in the original strand, U19, A20, and U21 are deleted, what peptide is produced? 1f. If, in the original strand, U21, A22, and U23 are deleted, what peptide is produced? Given the following mRNA: 5'-AUG-AAA-UCU-CCC-CGU-UGG-UAU-AUA-UUU-UGA-3'
Please answer all 1a. - 1f.
1a. Write the peptide coded by this mRNA strand. 1b. Write a different mRNA strand which codes for the same peptide, but has four point mutations. 1c. If, in the original strand, A5 is replaced by a G, and U7 is replaced by an C, what peptide is produced? 1e. If, in the original strand, U19, A20, and U21 are deleted, what peptide is produced? 1f. If, in the original strand, U21, A22, and U23 are deleted, what peptide is produced?
Please answer all 1a. - 1f.
1a. Write the peptide coded by this mRNA strand. 1b. Write a different mRNA strand which codes for the same peptide, but has four point mutations. 1c. If, in the original strand, A5 is replaced by a G, and U7 is replaced by an C, what peptide is produced? 1e. If, in the original strand, U19, A20, and U21 are deleted, what peptide is produced? 1f. If, in the original strand, U21, A22, and U23 are deleted, what peptide is produced? Please answer all 1a. - 1f.
1a. Write the peptide coded by this mRNA strand. 1b. Write a different mRNA strand which codes for the same peptide, but has four point mutations. 1c. If, in the original strand, A5 is replaced by a G, and U7 is replaced by an C, what peptide is produced? 1e. If, in the original strand, U19, A20, and U21 are deleted, what peptide is produced? 1f. If, in the original strand, U21, A22, and U23 are deleted, what peptide is produced?

Explanation / Answer

1a) Met K S P R W Y I F Stop

1b) based on the codon degeneracy, changing the 3rd nucleotide of a codon can code for the same amino acid.

the all possible codons for the above peptide sequence is

Methionine

Lysine

Serine

Proline

Arginine

Tryptophan

Tyrosine

Threonine

Phenyl alanine

Stop

AUG

AAA

AAG

UCU

UCA

UCG

UCC

AGC

AGU

CCC

CCA

CCG

CCU

CGU

CGA

CGG

CGC

AGA

AGG

UGG

UAU

UAC

AUA

AUG

AUC

AUU

UUU

UUC

UGA

UAG

UAA

The given mRNA sequence in shown in bold, and the alternate sequence with 4 point mutation (replacement mutation) can be:

5'-AUG-AAG-UCU-CCG-CGU-UGG-UAC-AUA-UUC-UGA-3'

this is one example. we can make many sequences for the abovesaid condition.

1c) replacing A5 by G and U7 by C: 5'-AUG-AGA-CCU-CCC-CGU-UGG-UAU-AUA-UUU-UGA-3'

AGA codes for Arginine and CCU codes for proline, the new peptide sequence will be:

Met R P P R W Y I F Stop

1e) deleting U19, A20, and U21 :5'-AUG-AAA-UCU-CCC-CGU-UGG- xxx-AUA-UUU-UGA-3'

since there is no frameshift, and an entire codon has been deleted, tyrosine will be removed and the new peptide sequence will be :Met K S P R W I F Stop

1f) deleting U21, A22,and U23 :

5'-AUG-AAA-UCU-CCC-CGU-UGG-Uax-xxA-UUU-UGA-3'

5'-AUG-AAA-UCU-CCC-CGU-UGG-UAA-UUU-UGA-3',

UAA is a stop codon, therefore, the peptide will be truncated as Met K S P R W Stop