Biology and Genetics

You are planning an experimental strategy for isolating your favorite gene and c

You are planning an experimental strategy for isolating your favorite gene and cloning it int o a bacterial vector. You decide to use the E. coli strain MM294 as your plasmid host…

You are planning to run a fermenter to produce antibiotics from fungi, using a f

You are planning to run a fermenter to produce antibiotics from fungi, using a fermentation broth that contains 15 wt% glucose, 6 wt% phosphate, 6 wt% nitrate, various trace nutri…

You are planning to start a new biotechnology company producing a recombinant pr

You are planning to start a new biotechnology company producing a recombinant protein KLV that stimulate antiviral responses. The cDNA encoding the protein (600 bp) and a bacteria…

You are planning your vacation in May and will be visiting friends who live in D

You are planning your vacation in May and will be visiting friends who live in Des Moines, Washington, a city south of Seattle on Puget Sound. Refer to the map being projected on …

You are playing a fun game of basketball with your friends. As you jump up for t

You are playing a fun game of basketball with your friends. As you jump up for the ball you land on another player’s foot and roll your ankle over. There are 4 motions of the ankl…

You are preparing a large scale bacterial virus (phage) lysate. In your refriger

You are preparing a large scale bacterial virus (phage) lysate. In your refrigerator you have 100 ml of a primary suspension of the bacterial virus to be amplified. The titer of t…

You are preparing to gibe medications to your patient. She has 10 oral medicatio

You are preparing to gibe medications to your patient. She has 10 oral medications due at 0900 plus eyedrops. You have verified the physician's orders with the MAR. There is one m…

You are presented with \'Nightshade\' a 7 year old, female spayed Doberman pinsc

You are presented with 'Nightshade' a 7 year old, female spayed Doberman pinscher with epistaxis. The dog first experienced epistaxis last night. It resolved after about 15 minute…

You are presented with a gram of a newly isolated animal virus. Electron microsc

You are presented with a gram of a newly isolated animal virus. Electron microscopy reveals the presence of a typical membrane surrounding the virus, and chemical analysis shows t…

You are presented with a patient, named Gerry, who has Down syndrome (trisomy 21

You are presented with a patient, named Gerry, who has Down syndrome (trisomy 21). You determine the nondisjunction event occurred in his mother, named Mary, during meiosis I. The…

You are presented with a patient, named Gerry, who has Down syndrome (trisomy 21

You are presented with a patient, named Gerry, who has Down syndrome (trisomy 21). You determine that the nondisjunction event occurred in his mother, named Mary, during meiosis I…

You are presented with the following data from a series of purification steps fo

You are presented with the following data from a series of purification steps for the enzyme comatose. Fill in the Blanks Below Given the purification schemed data show in problem…

You are prone to sunburn, but you doctor tells you not to apply your sunscreen u

You are prone to sunburn, but you doctor tells you not to apply your sunscreen until you have been outside for five minutes. Why? Select one: a. Skin pigmentation, as occurs when …

You are provided with a concentrated, purified, nucleic acid sample containing a

You are provided with a concentrated, purified, nucleic acid sample containing a single DNA molecule. (15 points) 9. (a) Describe how electrophoresis could have been used to prepa…

You are provided with the following information about a new receptor protein des

You are provided with the following information about a new receptor protein designated p15: p15 is an integral membrane protein the complete amino acid sequence of p15 contains 7…

You are purifying the enzyme Hexokinase (which converts Glc rightarrow Glc-6-PO_

You are purifying the enzyme Hexokinase (which converts Glc rightarrow Glc-6-PO_4) from rat liver cells. The figure shows your results from the various purfication steps. Both the…

You are purifying the enzyme Hexokinase (which converts Glc rightarrow Glc-6-PO_

You are purifying the enzyme Hexokinase (which converts Glc rightarrow Glc-6-PO_4) from rat liver cells. The figure shows your results from the various purification steps. Both th…

You are reading a label and the ingredients list includes pyridoxine as an addit

You are reading a label and the ingredients list includes pyridoxine as an additive. Which vitamin has been added to this food? 59) A) vitamin B12 B) vitamin B6 C) vi amin C D) vi…

You are ready to set up your reaction. You are provided with a stock of plasmid

You are ready to set up your reaction. You are provided with a stock of plasmid DNA that is at a concentration of 1mg/ml. You have stock enzyme buffer that is 10X (meaning it is t…

You are reconstructing the history of this area. 1 is the oldest event and 12 wo

You are reconstructing the history of this area. 1 is the oldest event and 12 would be the youngest. You have the info from the first question. You just need to insert the other u…

You are repeating Griffith\'s experiments. Label each experiment with the type o

You are repeating Griffith's experiments. Label each experiment with the type of bacteria, if any you would be able to isolate from the mouse after it had been injected, and wheth…

You are required to complete a 1-page (double spaced, 12pt. font, times new roma

You are required to complete a 1-page (double spaced, 12pt. font, times new roman) reflection paper turned in to the instructor via the dropbox in D2L. If you use the attachment o…

You are required to prepare standard standards for a calibration curve. All the

You are required to prepare standard standards for a calibration curve. All the glassware and pipettes your method requires have a tolerance (RSD) of 0.05%. You start working solu…

You are required to prepare standards for a calibration curve. All of the glassw

You are required to prepare standards for a calibration curve. All of the glassware and pipettes your method requires have a tolerance (RSD) of 0.15%. You start with a working sol…

You are required to produce a complete topic to sentence outline, an introductio

You are required to produce a complete topic to sentence outline, an introduction and ONE body paragraph on a topic selected from the following question: Topic: The Caribbean regi…

You are researching how our brains respond to music. 1. What general region (lob

You are researching how our brains respond to music. 1. What general region (lobe) of the brain are you interested in? 2. You want to know whether activation in the primary audito…

You are responsible for developing an exercise prescription for a post-CABG (cor

You are responsible for developing an exercise prescription for a post-CABG (coronary artery bypass graft) surgery patient who has just been referred to Phase II cardiac rehabilit…

You are responsible for your assigned microscope! Microscope Storage Proper prep

You are responsible for your assigned microscope! Microscope Storage Proper preparation of the microscope for storage reduces the chances of damage, and ensures that it will be re…

You are reviewing the record of Randy Robertson, a child seen by Dr. Carson, a p

You are reviewing the record of Randy Robertson, a child seen by Dr. Carson, a pediatrician. The physician has just determined that the child has hemophilia. In order to reach thi…

You are running a nursery for garden peas at LSU, and you have two pure-bred gar

You are running a nursery for garden peas at LSU, and you have two pure-bred garden pea strains: Yellow Wrinkled [YYrr] and Green Round [yyRR]. You know that Yellow is dominant to…

You are running your own lab and one of your primary research topics focuses on

You are running your own lab and one of your primary research topics focuses on corn and carbon allocation in seeds (kernels). Your previous research suggests that seeds with high…

You are scientist working for the USDA(United States Department of Agriculture)

You are scientist working for the USDA(United States Department of Agriculture) and are studying Aflatoxin B_1, a potent substance produced by Aspergillus on stored grain products…

You are screening a chemical for potential mutagenicity using the Ames test. You

You are screening a chemical for potential mutagenicity using the Ames test. You used a his – S. typhimurium bacterium for this test and obtained the following results:           …

You are screening for antibiotic resistant bacteria in the gut of a cow whose di

You are screening for antibiotic resistant bacteria in the gut of a cow whose diet is supplimented with antibiotics (a common practice in the USA). You take 1 ml of gut content an…

You are screening newborns to see if they have the progerin gene mutation or not

You are screening newborns to see if they have the progerin gene mutation or not.  Describe how you would use FISH as a diagnostic toolfor this disease. What technique could you u…

You are screening three new pesticides for potential mutagenicity using the Ames

You are screening three new pesticides for potential mutagenicity using the Ames test. Two his- strains resulting from either a frameshift or a transition mutation were used and p…

You are searching for new species that are highly tolerant to acidic conditions

You are searching for new species that are highly tolerant to acidic conditions and find a new single celled organism that you call WERID. The organism is ~5 um, does not have a c…

You are sequencing a protein. Previous work indicates that this protein consists

You are sequencing a protein. Previous work indicates that this protein consists of two polypeptide chains that are held together by a single disulfide bond. You perform two exper…

You are sequencing a protein. Previous work indicates that this protein consists

You are sequencing a protein. Previous work indicates that this protein consists of two polypeptide chains that are held together by a single disulfide bond. You perform two exper…

You are sequencing a protein. Previous work indicates that this protein consists

You are sequencing a protein. Previous work indicates that this protein consists of two polypeptide chains that are held together by a single disulfide bond. You perform two exper…

You are sequencing the genome of an organism brought back by Elon Musk\'s missio

You are sequencing the genome of an organism brought back by Elon Musk's mission to Mars. Below is part of the sequence that you think is important and you want to amplify it usin…

You are set to start a new earlier shift at your job. Your alarm clock goes off

You are set to start a new earlier shift at your job. Your alarm clock goes off at 8am (for the old shift) instead of 6am(for the new shift). You look toward your right to see the…

You are setting up an agarose gel and you accidentally plug in the electrodes ba

You are setting up an agarose gel and you accidentally plug in the electrodes backwards. That is likely to happen when you turn on the current? A. The instrument will short-circui…

You are sick in bed, at home. You\'re flipping aimlessly through the channels, t

You are sick in bed, at home. You're flipping aimlessly through the channels, trying to find something good on daytime television, and pause for a moment to see some chair-throwin…

You are sick: you have a high fever and have a sore throat. You go to the doctor

You are sick: you have a high fever and have a sore throat. You go to the doctor and tell them you think you have “strep throat”. The result comes back negative. The physician tel…

You are speeding down the highway going 85 in a 65mph speed limit zone and you p

You are speeding down the highway going 85 in a 65mph speed limit zone and you pass a police officer. Immediately your breathing rate increases, your heart rate increases and your…

You are standing at the edge fo a swimming pool and shining a laser pointer onto

You are standing at the edge fo a swimming pool and shining a laser pointer onto the water surface. The laser beam hits the water at an angle of 50.8 deg above the horizontal. Som…

You are standing on a sheet of ice that covers the football stadium parking lot

You are standing on a sheet of ice that covers the football stadium parking lot in Buffalo; there is negligible friction between your feet and the ice. A friend throws you a 0.395…

You are starting a new biotech company and want to use phage as the focus of you

You are starting a new biotech company and want to use phage as the focus of your company’s efforts. Which of the following ideas will convince your capital venturists to invest i…

You are starting with an individual who has a total fluid volume of 3 liters (L)

You are starting with an individual who has a total fluid volume of 3 liters (L) with an osmolarity of 280 milliosmoles (mOsM; mOsm/L). The fluid is divided among the body compart…