Biology and Genetics

4. You are a new researcher in your lab and want to make your mark by determinin

4. You are a new researcher in your lab and want to make your mark by determining the function of unknown human genes. You are looking through unannotated parts of the human genom…

4. You are a pharmacist in-training at a local hospital and are told to prepare

4. You are a pharmacist in-training at a local hospital and are told to prepare an intravenous (IV) bag of antibiotics in order to treat a patient with bacterial sepsis (i.e., inf…

4. You are a research assistant in a lab that studies nucleic acids. Your adviso

4. You are a research assistant in a lab that studies nucleic acids. Your advisor gave you four tubes for analysis. Each of these tubes differs in its contents by the source of it…

4. You are a researcher working on drug development and have synthesized a large

4. You are a researcher working on drug development and have synthesized a large number of molecules. After treating cultured human cells with the different drugs, you discover th…

4. You are an ecologist studying the recovery of California condors in the area

4. You are an ecologist studying the recovery of California condors in the area around      the Grand Canyon. You want to conduct a survey to determine the current      population…

4. You are at a spa and are presented with a mud mask. Being a very particular e

4. You are at a spa and are presented with a mud mask. Being a very particular engineering geologist, you say that you only use mud masks that have a saturated unit weight (?m) th…

4. You are attempting to clone an EcoRI fragment into an EcoRI-restricted vector

4. You are attempting to clone an EcoRI fragment into an EcoRI-restricted vector treated with CIP, and you obtain the following results on your transformation plates (with Amp): #…

4. You are interested in studying the transcriptional regulation of a gene expre

4. You are interested in studying the transcriptional regulation of a gene expressed in the gut called Smrtl. The promoter for Smrtl contains binding sites for two transcriptional…

4. You are new to the lab and you want to impress your fellow graduate students

4. You are new to the lab and you want to impress your fellow graduate students so you have volunteered to make the next batch of electroporation competent E. coli cells. You foll…

4. You are out in the field and stumble upon an aesthetically pleasing rock; you

4. You are out in the field and stumble upon an aesthetically pleasing rock; your colleague tells you that it is part of a large mafic dike and it has been dated using U-Pb zircon…

4. You are starting a research program in a Drosophila lab, A previous students

4. You are starting a research program in a Drosophila lab, A previous students did a mutagenesis project and have extra legs, and you are interested in the genetics of this strai…

4. You are supplied with the following information about a DNA molecule: . The m

4. You are supplied with the following information about a DNA molecule: . The molecular weight of a typical nucleotide is 660 Da. . The linear density of double stranded DNA is 4…

4. You are working red flowers. Y F2 phenotypes: 777 red and 219 yellow. on the

4. You are working red flowers. Y F2 phenotypes: 777 red and 219 yellow. on the genetics of flower color in a plant that breeds true for either yellow or ou begin by crossing yell…

4. You get tired of watching the hypnotic motion of the backpack bouncing up and

4. You get tired of watching the hypnotic motion of the backpack bouncing up and down. So you take it off the bungee cord and hang it from a rope 2.00 m long rope instead. A breez…

4. You have 26 ul of RNA that you would like to load on a gel. You need to add a

4. You have 26 ul of RNA that you would like to load on a gel. You need to add a gel loading buffer (which contains glycerol and a dye) so that the RNA sample sinks into the well.…

4. You have generated different mutant Ag-presenting cell lines. Each mutant cel

4. You have generated different mutant Ag-presenting cell lines. Each mutant cell line cannot make a functional version of the indicated protein. For each cell line, indicate whet…

4. You have isolated a yeast protein involved in transcriptional regulation and

4. You have isolated a yeast protein involved in transcriptional regulation and found a stretch of basic amino acids in the middle of this protein. You suspect that this sequence …

4. You have just graduated from nursing school and started a job at the local co

4. You have just graduated from nursing school and started a job at the local correctional facility in the health clinic. Your first client is a female who has just been transferr…

4. You have two heterozygous solid black cats. Complete a punnett square illustr

4. You have two heterozygous solid black cats. Complete a punnett square illustrating this cross. What fraction of the offspring will have solid orange coats? 5. You have a hetero…

4. You have two patients with the symptoms of meningitis. You examine the CSF fr

4. You have two patients with the symptoms of meningitis. You examine the CSF from both patients, conduct a gram stain and come up with the following images. a. What types of cell…

4. You perform a PCR reaction using the following template DNA and primers. What

4. You perform a PCR reaction using the following template DNA and primers. What size(s) of products do you expect? Explain vour answer. Primer: 5'-ctctgggctc agagcagccc-3 Primer:…

4. You perform a PCR reaction using the following template DNA and primers. What

4. You perform a PCR reaction using the following template DNA and primers. What size(s) of products do you expect? Explain vour answer. Primer: 5'-ctctgggctc agagcagccc-3 Primer:…

4. You perform a PCR reaction using the following template DNA and primers. What

4. You perform a PCR reaction using the following template DNA and primers. What size(s) of products do you expect? Explain vour answer. Primer: 5'-ctctgggctc agagcagccc-3 Primer:…

4. You perform a PCR reaction using the following template DNA and primers. What

4. You perform a PCR reaction using the following template DNA and primers. What size(s) of products do you expect? Explain vour answer. Primer: 5'-ctctgggctc agagcagccc-3 Primer:…

4. You perform a series of experiments on the synthesis of the primary hormone p

4. You perform a series of experiments on the synthesis of the primary hormone prolactin, which is a single polypeptide chain 199 amino acids long. The mRNA coding for prolactin i…

4. You propose a set of experiments to test whether present-day chloroplasts ori

4. You propose a set of experiments to test whether present-day chloroplasts originated from a single or multiple endosymbiotic events. Which set of experiments below would provid…

4. You take total cell lysate, and use immunoprecipitation to purify an enzyme o

4. You take total cell lysate, and use immunoprecipitation to purify an enzyme of interest. The purified enzyme has HIGH specific activity, and shows a single band (56kD) on an SD…

4. You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate

4. You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to use? Which would be inappropriate? Explain your answer. 5. You want to make a genomic library w…

4. You will estimate the quantity of DNA present in the pUC18 DNA prep by gel ba

4. You will estimate the quantity of DNA present in the pUC18 DNA prep by gel band brightness. Compare your linear plasmid band in the HindIII digest lane to the -HindIII marker. …

4. You\'ve determined that a rare blood cancer drastically alters leukocyte (WBC

4. You've determined that a rare blood cancer drastically alters leukocyte (WBC) mechanical behavior. The viscoelastic behavior of these WBCs is appropriately modeled by a simple …

4. Your friend is studying a secreted protein called Inp1, Inp1 is produced as a

4. Your friend is studying a secreted protein called Inp1, Inp1 is produced as a precursor form called pro-Inp1. The maturation of Inp1 requires that a short N-terminal segment be…

4. Your friend is studying a secreted protein called Inp1. Inp1 is produced as a

4. Your friend is studying a secreted protein called Inp1. Inp1 is produced as a precursor form called pro-Inp1. The maturation of Inp1 requires that a short N-terminal segment be…

4. Your friend is studying a secreted protein called Inp1. Inp1 is produced as a

4. Your friend is studying a secreted protein called Inp1. Inp1 is produced as a precursor form called pro-Inp1. The maturation of Inp1 requires that a short N-terminal segment be…

4. You’ve run an interrupted mating conjugation experiment with Hfr cells that a

4. You’ve run an interrupted mating conjugation experiment with Hfr cells that are hmm+ omg+ poo+ and streptomycin resistant (strR). Your recipient cell is F hmm omg poo and strep…

4. Zymomonas mobilis is one of the only organisms that can only metabolize gluco

4. Zymomonas mobilis is one of the only organisms that can only metabolize glucose through the Entner-Doudoroff pathway and does not contain any type of electron transport chain. …

4. [20 points total] In a sample of a population, 100 individuals were genotyped

4. [20 points total] In a sample of a population, 100 individuals were genotyped using a molecular technique that produced the following banding patterns on an electrophoretic gel…

4. \"Ballpark\" estimates of time scales for melting an ice sheet Even for a com

4. "Ballpark" estimates of time scales for melting an ice sheet Even for a complicated system like an ice sheet, it can be useful to get a rough idea of the range of possible beha…

4. a. (2 pts) There is a herd of elephants that has trunks that vary in length f

4. a. (2 pts) There is a herd of elephants that has trunks that vary in length from 4 to 7 feet. One of their primary sources offood is leaves on trees and bushes. Only elephants …

4. a. Romeo, a promising thoroughbred stallion, was recently retired to stud. Hi

4. a. Romeo, a promising thoroughbred stallion, was recently retired to stud. His first five foals have just completed their maiden races, averaging 2.5 seconds faster than their …

4. a. Which steps in gluconeogenesis DO NOT occur in the cytoplasm? b. Gluconeog

4. a. Which steps in gluconeogenesis DO NOT occur in the cytoplasm? b. Gluconeogenesis can synthesize glucose because it is energetically favorable in the cell. It is energeticall…

4. farmers are talking together about the future. One is hopeful that people wil

4. farmers are talking together about the future. One is hopeful that people will start taking care of the land differently. Another says, “Don’t fool yourself… It’s just not in o…

4. ho is credited with first providing conclusive evidence of species extinction

4. ho is credited with first providing conclusive evidence of species extinction? A) Charles Lyell B) Georges Cuvier C) Alfred Russel Wallace 5. Who is credited for the discovery …

4. on a typical, day-to-day basis, the liver performs gluconeogenesis at a low r

4. on a typical, day-to-day basis, the liver performs gluconeogenesis at a low rate. But the liver can perform gluconeogenesis at a high rate, when necessary. (a) Describe glucone…

4. which of the following is true about these exotoxin-caused diseases? a. dipht

4. which of the following is true about these exotoxin-caused diseases? a. diphtheria and botulism are infections, but tetanus is an intoxication b. botulism and tetanus are infec…

4.(12 pts) Controlled release of chemicals is important in agriculture, especial

4.(12 pts) Controlled release of chemicals is important in agriculture, especially for insect control. One common example involves the use of pheromones, attractants released by i…

4.) A DNA copy of an mRNA molecule iscalled A) dDNA B) mRNA C) rDNA D) cDNA E)rn

4.) A DNA copy of an mRNA molecule iscalled A) dDNA B) mRNA C) rDNA   D) cDNA E)rnaDNA 5.) _______blots are used to studyDNA fragments, while _______ blots are used to study RNAfr…

4.) After a normal (not diabetic) person has eaten a meal and the blood glucose

4.) After a normal (not diabetic) person has eaten a meal and the blood glucose level has increased, A.) The level of the hormone insulin increases in the blood, while the amount …

4.) Explain the difference between the following point mutations: Silent Missens

4.) Explain the difference between the following point mutations: Silent Missense Nonsense 5.) Explain the difference between a conditional lethal mutation, suppressor mutation an…

4.) GREEN ENERGY SOURCES Petroleum and coal can meet the energy demands of the U

4.) GREEN ENERGY SOURCES Petroleum and coal can meet the energy demands of the United States for decades to come, but are causing costly climate changes locally and globally due t…

4.) PLATE TECTONICS 10 Mt. Everest 8850 meters Land 29.296 Avg. elevation of and

4.) PLATE TECTONICS 10 Mt. Everest 8850 meters Land 29.296 Avg. elevation of and 840 meters level Avg. depth of oceans 3729 meters margin Deep ocearn Trenches Ocean-70.8% 10 Maria…