Biology and Genetics

You performed a co-immunoprecipitation experiment to identify cellular proteins

You performed a co-immunoprecipitation experiment to identify cellular proteins associated with protein X. For the experiment, you used antibodies against protein X that were immo…

You performed a miniprep and put the DNA into Tube 1, containing a total volume

You performed a miniprep and put the DNA into Tube 1, containing a total volume of 450l. You then took 10l of the DNA from Tube 1 and added it to 70l of water in Tube 2. You then …

You performed a soil extraction (1 mL peptone solution for 1 g soil) as the extr

You performed a soil extraction (1 mL peptone solution for 1 g soil) as the extracting solution. A dilution and plating technique is performed using the spread plate method by spr…

You performed breeding experiments on a newly discovered species of unicorn. the

You performed breeding experiments on a newly discovered species of unicorn. the horns came in three colors: white, black and gray. A single gene controls color. When you crossed …

You performed gel electrophoresis and this is how your gel came out 3m You refer

You performed gel electrophoresis and this is how your gel came out 3m You refer to your notebook to see what sample was loaded into each well. Going from top to bottom: Lane 1 St…

You performed gel electrophoresis and this is how your gel came out 3m You refer

You performed gel electrophoresis and this is how your gel came out 3m You refer to your notebook to see what sample was loaded into each well. Going from top to bottom: Lane 1 St…

You performed matings with other flies from question 3 and found some unusual re

You performed matings with other flies from question 3 and found some unusual results. The 1600 F2 progeny are the result of crossing the F1 progeny to each other. P1: thick wings…

You performed one of the four chain-termination reactions using ddATP, dCTP, dGT

You performed one of the four chain-termination reactions using ddATP, dCTP, dGTP, and dTTP in the reaction? Your sequencing did not work. Please explain why. On the get below, yo…

You performed the following tests to identify your unknown bacterial species, an

You performed the following tests to identify your unknown bacterial species, and obtained these results. Unfortunately, one of your culture tubes was contaminated during inoculat…

You pick one o following nutrient pools. (Each row and column is a separate pool

You pick one o following nutrient pools. (Each row and column is a separate pool. So for e alanine, tryptophan and phenylalanine, while pool 4 contains alanine, guanine and threon…

You pick one of your auxotrophic mutants and test it for what nutrient it needs

You pick one of your auxotrophic mutants and test it for what nutrient it needs to grow. So that you don't have to do 20 different plates, you use a pooled nutrient strategy. Yeas…

You pick one of your auxotrophic mutants and test it for what nutrient it needs

You pick one of your auxotrophic mutants and test it for what nutrient it needs to grow. So that you don't have to do 20 different plates, you use a pooled nutrient strategy. Yeas…

You place 4 fruit flies into each of the 100 vials: one black-bodied male fruit

You place 4 fruit flies into each of the 100 vials: one black-bodied male fruit fly (bb) 2 homozygous wild-type females (BB) and one homozygouse wild-type male (BB). You allow the…

You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA

You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase ? for 1 hour (0 enzyme units, 0.25 enzyme unit…

You plan to buy a exist220,000 house with a 20 year mortgage with a 4.8% nominal

You plan to buy a exist220,000 house with a 20 year mortgage with a 4.8% nominal annual rate (=A.P.R.) Payments are monthly, interest is monthly compounded, and you did not make a…

You plan to identify the relative position of sequences recognized by the restri

You plan to identify the relative position of sequences recognized by the restriction enzymes PstI and EcoRV on a circular plasmid. The restriction sites are the following: PstI: …

You plan to redo the test cross, and need to decide how many F1 offspring to loo

You plan to redo the test cross, and need to decide how many F1 offspring to look at. You know that if you see any wrinkled offspring, the unknown was Rr. However, if you see only…

You plan to take a trip to the moon. Since you do not have a traditional spacesh

You plan to take a trip to the moon. Since you do not have a traditional spaceship with rockets, you will need to leave the earth with enough speed to make it to the moon. Some in…

You plan to use mutagenesis to add a restriction site to facilitate cloning of y

You plan to use mutagenesis to add a restriction site to facilitate cloning of your favorite gene into an expression vector. Which of the primer pairs listed could be used to plac…

You plated an unknown yeast auxotrophic mutant on MD media and left it in the in

You plated an unknown yeast auxotrophic mutant on MD media and left it in the incubator for a week. At the end of the week, you had no growth on the plate. What is the MOST LIKELY…

You pour 160 g hot coffee at 78.7°C and some cold cream at 7.50°C to a 115-g cup

You pour 160 g hot coffee at 78.7°C and some cold cream at 7.50°C to a 115-g cup that is initially at a temperature of 22.0°C. The cup, coffee, and cream reach an equilibrium temp…

You prepare a patient for the sterile procedure. Which of the following statemen

You prepare a patient for the sterile procedure. Which of the following statements by the patient best demonstrates understanding of the need for sterile equipment and aseptic pro…

You prepare a patient for the sterile procedure. Which of the following statemen

You prepare a patient for the sterile procedure. Which of the following statements by the patient best demonstrates understanding of the need for sterile equipment and aseptic pro…

You prepare bacterial cell extracts by lysing the cells and removing insoluble d

You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation. These extracts provide all the protiens required for DNA repication. The …

You prepared 2 plasmids by a mini-prep method, diluted the final solutions 1:50,

You prepared 2 plasmids by a mini-prep method, diluted the final solutions 1:50, and using a spectrophotometer and standard cuvette, you determined the UV absorbances at 260 nm an…

You prepared a tuna sandwich st 2pm, accidentally adding 100 salmonallae to it.

You prepared a tuna sandwich st 2pm, accidentally adding 100 salmonallae to it. You know that 30,000 microbes are necessary to start infection and the replication rate of this par…

You previously diagnosed a patient with a form of cancer that seemed to have H3K

You previously diagnosed a patient with a form of cancer that seemed to have H3K9me3 modifications on the histones at the p53 gene. You gave this patient:a H3K9me3 methyltransfera…

You propose a null hypothesis based on your F1 fruit flies; You are a scientist

You propose a null hypothesis based on your F1 fruit flies; You are a scientist trying to understand how wing shape is inherited in fruit flies. You have flies that are pure-breed…

You provide nasopharyngeal suction for the patient and obtain a moderate amount

You provide nasopharyngeal suction for the patient and obtain a moderate amount of thick secretion. After allowing the patient to recovery, you reassess the patient's respiratory …

You purified fragments of nuclear envelope from human fibroblasts by biochemical

You purified fragments of nuclear envelope from human fibroblasts by biochemical fractionation. You first decide to use electron microscopy to test for the presence of the nuclear…

You purified protein X via affinity chromatography (no defiltration step perform

You purified protein X via affinity chromatography (no defiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-P…

You purified protein X via affinity chromatography (no defiltration step perform

You purified protein X via affinity chromatography (no defiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-P…

You purified protein X via affinity chromatography (no diafiltration step perfor

You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-…

You purify Yet Another Enzyme (YAB) and perform still more Michaelis-Menten kine

You purify Yet Another Enzyme (YAB) and perform still more Michaelis-Menten kinetics experiments. You first test your purified enzyme with its substrate (uninhibited), then with e…

You ran your uncut plasmid DNA on a gel in Molecu lar Biology (Part II). Downloa

You ran your uncut plasmid DNA on a gel in Molecu lar Biology (Part II). Download your gel picture from the Molecular- III folder for your instructor/lab section on BOX and interp…

You read a report suggesting that E. coli expresses a protein repressor of the l

You read a report suggesting that E. coli expresses a protein repressor of the lactasegene. The repressor binds directly to the DNA and could be responsible for the absence of lac…

You read in the news that two elderly people have died of apparent food poisonin

You read in the news that two elderly people have died of apparent food poisoning from tainted meat. The state epidemiologist said there has been a recent outbreak of a disease wi…

You recall reading in the medical literature about a dramatic new drug treatment

You recall reading in the medical literature about a dramatic new drug treatment for sickle cell anemia, and you?d like to try it on this patient. The drug is hydroxyurea, and is …

You receive a case to process containing a sealed manila envelope with the the s

You receive a case to process containing a sealed manila envelope with the the subject’s standard sample (buccal swabs) as well as a separate envelope containing a shirt with heav…

You receive data back from the Qubit showing that your total RNA concentration i

You receive data back from the Qubit showing that your total RNA concentration is 2.5 ug/mL. The sample run on the Qubit was diluted 100-fold, so you must correct for this to dete…

You received no credit for this question in the previous attempt. View previous

You received no credit for this question in the previous attempt. View previous Which of the following statements is correct about antigen presentation by class I and class II MHC…

You received no credit for this question in the previous ettempt. 8 Carbonic Anh

You received no credit for this question in the previous ettempt. 8 Carbonic Anhydrase Carbonic anhydrase [CAL en enzyme widely present in plant and animal tissues, cateyzes the r…

You received partial credit in the previous attempt ic to the correct type of an

You received partial credit in the previous attempt ic to the correct type of animal. Amphibian Most reptiles, birds, and mammals surface area in the lung is much less air is forc…

You received partial credit in the previous attempt. Vlev 3 Complex carbohydrate

You received partial credit in the previous attempt. Vlev 3 Complex carbohydrates Indicate whether each statement about polysaccharides is True or False. 1. Polysaccharides are lo…

You recently accepted a management position at a home health agency. You directl

You recently accepted a management position at a home health agency. You directly manage three team leads (Registered Nurses with management experience), each with eight Certified…

You recently discovered four new chemical mutagens (#1 - #4), but you do not kno

You recently discovered four new chemical mutagens (#1 - #4), but you do not know what type(s) of mutations these chemicals induce. You exposed E. coli to each chemical individual…

You recently discovered four new chemical mutagens (#1- #4), but you do not know

You recently discovered four new chemical mutagens (#1- #4), but you do not know what type(s) of mutations these chemicals induce. You exposed E. coli to each chemical individuall…

You recently identified a new compound, TX32, present in e-cigarette vapor that

You recently identified a new compound, TX32, present in e-cigarette vapor that you suspect causes mitochondrial dysfunction. Your preliminary data from Part 1 indicated that TX32…

You recently identified a new mutation in Xenopus. We don’t know which gene is m

You recently identified a new mutation in Xenopus. We don’t know which gene is mutated, but when you observe the mutant embryo it appears to gastrulate, elongate, and make it thro…

You recently isolated two separate strains of yeast which have growth defects wh

You recently isolated two separate strains of yeast which have growth defects when grown in uracil - lacking media. Normal (wildtype) yeast do not require uracil for growth. Mutan…