Biology and Genetics

You have introduced a series of mutations in mice that impair synaptonemal compl

You have introduced a series of mutations in mice that impair synaptonemal complex (SC) formation during prophase I of meiosis. The mutations do not affect pre-synaptic pairing or…

You have ion exchange, gel filtration and NAD-affinity columns available to you.

You have ion exchange, gel filtration and NAD-affinity columns available to you. Which ones would you use to purify protein A away from the other three proteins? Sketch the elutio…

You have isolated 10 mutant yeast strains defective in synthesis of leucine (num

You have isolated 10 mutant yeast strains defective in synthesis of leucine (numbered 1- 10). (Mutants 7 and 9 got contaminated and were discarded). These Leu- mutants do not grow…

You have isolated 2 different strains of bacteria (bacteria A and bacteria B) bo

You have isolated 2 different strains of bacteria (bacteria A and bacteria B) both of which uptake and metabolize the monosaccharide galactose to various degrees. You suspect that…

You have isolated DNA from three different cell types of an organism, determined

You have isolated DNA from three different cell types of an organism, determined the relative DNA content for each type, and plotted the results on the graph shown in the figure. …

You have isolated DNA from three different cell types of the same organism deter

You have isolated DNA from three different cell types of the same organism determined the relative DNA content for each type, and plotted the results on the graph shown in the fig…

You have isolated Protein X with a known function Y. Based on sequencing; you be

You have isolated Protein X with a known function Y. Based on sequencing; you believe that it is a double pass transmembrane protein at the ER. What can you do experimentally to t…

You have isolated a 10,500 bp plasmid from bacteria. This plasmid is a closed ci

You have isolated a 10,500 bp plasmid from bacteria. This plasmid is a closed circular double stranded DNA molecule characterized by a superhelical density = -0.05. The superhelic…

You have isolated a Gram positive soil bacterium, which you have named Gretzykri

You have isolated a Gram positive soil bacterium, which you have named Gretzykrinia olierensis. You hypothesize that these bacteria can undergo natural transformation. To test thi…

You have isolated a bacterial transporter that transports Mg2+ and Ca2+ in oppos

You have isolated a bacterial transporter that transports Mg2+ and Ca2+ in opposite directions. You produce lipid vesicles that contain this Mg2+ -Ca2+ pump as The sole membrane p…

You have isolated a clinical sample and carried out a gram stain on the bacteria

You have isolated a clinical sample and carried out a gram stain on the bacteria in hopes to identify what is causing the illness in your patient. The gram stain showed a gram-pos…

You have isolated a gene from a self incompatible plant, Solanum sofaaceae, bett

You have isolated a gene from a self incompatible plant, Solanum sofaaceae, better known as the "couch potato plant." You show the gene co-segregates with the S locus and that pla…

You have isolated a mixture of GST isoenzymes from rat liver tissue. You know th

You have isolated a mixture of GST isoenzymes from rat liver tissue. You know that the mixture contains the following GST classes: GST alpha 2 GST alpha 3 GST omega 1 GST kappa 1 …

You have isolated a new nuclear protein, whose expression level is signficantly

You have isolated a new nuclear protein, whose expression level is signficantly increased in cancer. Careful sequence analysis reveals the following sequence in the N-terminus of …

You have isolated a novel species of yeast from the dumpster behind Appalachian

You have isolated a novel species of yeast from the dumpster behind Appalachian Mountain Brewery. You would like to sequence the genome of this yeast species for further analysis.…

You have isolated a number of E.coli strains with mutations in genes involved in

You have isolated a number of E.coli strains with mutations in genes involved in lactose utilization (lac operon genes). Depending on the particular mutation they carry, the diffe…

You have isolated a primary signaling factor from the blood of a space alien, wh

You have isolated a primary signaling factor from the blood of a space alien, which you name XFF (for X-files factor). You also have isolated various types of alien cells which ap…

You have isolated a protein within the nucleus for further study. What would you

You have isolated a protein within the nucleus for further study. What would you expect to find with respect to this protein? A) a signal recognition protein sequence B) highly ch…

You have isolated a protein, but now need to determine the amino acid sequence.

You have isolated a protein, but now need to determine the amino acid sequence. Your results are below: After one cycle of Edman degradation the phenylthiohydantoin, PTH, derivati…

You have isolated a sequence-specific DNA-binding protein (Protein X) with the f

You have isolated a sequence-specific DNA-binding protein (Protein X) with the following primary sequence: This protein binds as a homodimer, and sequence homology studies with ot…

You have isolated a strain of temperature-sensitive mutant yeast that divides no

You have isolated a strain of temperature-sensitive mutant yeast that divides normally at 25°C. However, at 37°C, the mutant pauses in the cell cycle right before M phase. These m…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocated proteins into mitochondria. So far you have eliminated any of…

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate proteins into mitochondria. So far you have eliminated any of …

You have isolated a temperature-sensitive mutant that, at the restrictive temper

You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate proteins into mitochondria. So far you have eliminated any of …

You have isolated a tubular segment of rat nephron and introduced a solution of

You have isolated a tubular segment of rat nephron and introduced a solution of known composition into it. When you compare the fluid inside the tube collected at the end of the s…

You have isolated an epithelial cell line which is unresponsive to EGF (normal e

You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell lines require EGF in the culture media for growth). You suspect that EGF rec…

You have isolated an epithelial cell line which is unresponsive to EGF (normal e

You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell lines require EGF in the culture media for growth). You suspect that EGF rec…

You have isolated an epithelial cell line which is unresponsive to EGF (normal e

You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell lines require EGF in the culture media for growth). You suspect that EGF rec…

You have isolated an epithelial cell line which is unresponsive to EGF (normal e

You have isolated an epithelial cell line which is unresponsive to EGF (normal epithelial derived cell lines require EGF in the culture media for growth). You suspect that EGF rec…

You have isolated an intact preparation of mitochondria, and add malate and ADP

You have isolated an intact preparation of mitochondria, and add malate and ADP + Pi. You observe the consumption of oxygen and the simultaneous production of ATP. Starting with t…

You have isolated an octopeptide from a rare fungus. This peptideprevents baldne

You have isolated an octopeptide from a rare fungus. This peptideprevents baldness and you wish to determine its amino acidsequence. The composition is Lys2 , Asp, Tyr, Phe, Gly,S…

You have isolated an organi sm from the urine of Patient X, a 24 year old female

You have isolated an organi sm from the urine of Patient X, a 24 year old female. It is showing amma hemolysis on blood agar and the col onies are large with an entire margin and …

You have isolated an organism from the urine of Patient X, a 24 year old female.

You have isolated an organism from the urine of Patient X, a 24 year old female. It is showing gamma hemolysis on blood agar and the colonies are large with an entire margin and a…

You have isolated different recombinant cDNA clones from pancreas mRNA. Based on

You have isolated different recombinant cDNA clones from pancreas mRNA. Based on their DNA sequence, you suspect the cDNA clones encode a protein that binds to the promoter of an …

You have isolated double stranded DNA from a virus and measured the relative amo

You have isolated double stranded DNA from a virus and measured the relative amount of deoxyadenosine and deoxycytidine in the DNA sample. You denote the two strands in the molecu…

You have isolated five different homozygous recessive mutant lines that all prod

You have isolated five different homozygous recessive mutant lines that all produce the same phenotype, and now wish to determine if the mutations affect the same gene. To do this…

You have isolated intact mitochondria from cardiac tissue. Assume all proteins a

You have isolated intact mitochondria from cardiac tissue. Assume all proteins and cofactors (ADP, P, NAD+, FAD etc.) necessary for electron transport and oxidative phosphorylatio…

You have isolated intact mitochondria from cardiac tissue. Assume all proteins a

You have isolated intact mitochondria from cardiac tissue. Assume all proteins and cofactors (ADP, Pi NAD+, FAD etc.) necessary for electron transport and oxidative phosphorylatio…

You have isolated mRNA corresponding to a protein kinase involved in human cance

You have isolated mRNA corresponding to a protein kinase involved in human cancer. Your ultimate goal is to study the properties of this kinase in an enzymatic assay to understand…

You have isolated several different temperature sensitive mutant ycast strains t

You have isolated several different temperature sensitive mutant ycast strains that stop proliferating when shifted from 25 degree C to 37 degree C. You analyze these mutant strai…

You have isolated several different temperature sensitive mutant yeast strains t

You have isolated several different temperature sensitive mutant yeast strains that stop proliferating when shifted from 25 degree C to 37 degree C. You analyze these mutant strai…

You have isolated several different temperature sensitive mutant yeast strains t

You have isolated several different temperature sensitive mutant yeast strains that stop proliferating when shifted from 25 degrees C to 37 degrees C. you analyze these mutant str…

You have isolated six rll-deletion mutants of bacteriophage T4 (a-f) that lie wi

You have isolated six rll-deletion mutants of bacteriophage T4 (a-f) that lie within gene rllA. You coinfect E. coli strain B in all possible pairwise combinations and plate the r…

You have isolated the gene (XA) that codes for protein X from species A and you

You have isolated the gene (XA) that codes for protein X from species A and you want to determine if a gene encoding an identical protein is present in the genome of species B, i.…

You have isolated the gene for a protein, nuculin (a protein that some people be

You have isolated the gene for a protein, nuculin (a protein that some people believe resides in the nuculus) that is normally localized to the nucleus. You locate in the gene the…

You have isolated the gene for a protein, nuculin, that is normallylocalized to

You have isolated the gene for a protein, nuculin, that is normallylocalized to the nucleus. You locate in the gene the portion of thecoding sequence that codes for the nuclear lo…